the results of gel electrophoresis are shown below

the results of gel electrophoresis are shown below

the results of gel electrophoresis are shown below

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The results of gel electrophoresis are shown below. What can you determine?

the results of gel electrophoresis are shown below
the results of gel electrophoresis are shown below

about the DNA from looking at the results of this test?
electrode
A. Strand 1 is the smallest
B. Strand 2 is larger than strand 3
C. Strand 3 is larger than strand 4
D. Strand 4 is the smallest

ANSWER: A

Gel electrophoresis is an amazing technique that allows for DNA separation based on size (as in, length). Using electricity (and keeping in mind that DNA has a negative charge because of the phosphate groups), the shortest pieces will migrate the farthest.So we can tell that the pieces are in ascending order of size. 4>3>2>1, in length. A. Strand 1 is the smallest.

the results of gel electrophoresis are shown below
the results of gel electrophoresis are shown below

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How Does a Circular Plasmid DNA Run During Gel Electrophoresis?

The gel electrophoresis conditions (including the presence of ethidium bromide, gel concentrations, electric field strength, temperature, and ionic strength of the electrophoresis buffer) may affect the mobility of the plasmid DNA. Due to the net-like nature of agarose gel, circular plasmid DNA is caught up easier in the agarose mesh.

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The electrophoretic trapping is a balance between the electrophoretic force (pulling the circular plasmid DNA against the trap) and diffusion (allowing the circular plasmid DNA to escape a trap). So, large circular molecules have a greater chance to get trapped than smaller DNA. Supercoiled DNA are more difficult to trap due to the small size of the twisted DNA.

 

The results of gel electrophoresis are shown below What can you determine about the DNA from looking at the results of this test?

Strand 4 is the largest.


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the results of gel electrophoresis are shown below
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